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THUNDER™ Phospho-SMAD2 (S465/S467) TR-FRET Cell Signaling Assay Kit

2019-12-13

Price range: USD$409.00 through USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SMAD2 (S465/S467) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: this improved Phospho-SMAD2 (S465/S467) Assay Kit uses a new antibody pair that significantly improves the assay performance.

Product Citation: PubMed 35664796

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Stimulation of Phospho-SMAD2-New vs Old Kit
Data show the superior performance of the new Phospho-SMAD2 Assay compared to the previous version of the assay.
Inhibition of Phospho-SMAD2-New vs Old Kit
Data show the superior performance of the new Phospho-SMAD2 Assay compared to the previous version of the assay.
Stimulation of Phospho-SMAD2 (S465/S467) in HeLa cells
Data show that treatment of A549 cells with TGF-β1 stimulates phosphorylation of SMAD2 at S465/S467.
Inhibition of Phospho-SMAD2 (S465/S467) in HeLa cells
Data show that treatment of A549 cells with SB 431542 inhibits phosphorylation of SMAD2 at S465/S467 by TGFβ.
Z'-factor determination in HeLa cells
The Z’-factor value of 0.82 indicates that the assay is robust and suitable for HTS.
A549 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.