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THUNDER™ Phospho-RPS6 (S240/S244) TR-FRET Cell Signaling Assay Kit

2019-12-13

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Ribosomal Protein S6 (S240/S244) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-RPS6 (S240/S244) in MCF7 cells
Data show that treatment of MCF7 cells with insulin stimulates phosphorylation of RPS6 at S240/S244
Inhibition of Phospho-RPS6 (S240/S244) in MCF7 cells
Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of RPS6 at S240/S244 by insulin
Z'-factor determination in MCF7 cells
The Z’-factor value of 0.64 indicates that the assay is robust and suitable for HTS
MCF7 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values