THUNDER™ Total NF-kB TR-FRET Cell Signaling Assay Kit

2019-12-13

View technical data sheet

$409.00 – $8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total NF-κB (both phosphorylated and non-phosphorylated NF-κB) in cell lysates. This kit can be used to normalize data obtained with the Phospho-NF-κB (S536) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: N/A Category: NF-κB

Size

Clear

Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-NF-kB (S536) in HeLa cells

Data show that treatment of HeLa cells with TNFα stimulates phosphorylation of NF-kB at S536, but does not affect the levels of total NF-kB

Inhibition of Phospho-NF-kB (S536) in HeLa cells

Data show that treatment of HeLa cells with IKK-16 inhibits phosphorylation of NF-kB at S536 by TNFα, but does not affect the levels of total NF-kB

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Total NF-kB TR-FRET Cell Signaling Assay Kit

Additional information

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Total NF-kB TR-FRET Cell Signaling Assay Kit

Additional information

Related products

THUNDER™ Phospho-NF-kB (S536) + Total NF-kB TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-NF-kB (S536) TR-FRET Cell Signaling Assay Kit

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS