THUNDER™ Phospho-GSK3β (S9) TR-FRET Cell Signaling Assay Kit

2019-12-13

View technical data sheet View generic user manual

$409.00 – $8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-GSK3β (S9) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: N/A Category: GSK3β

Size

Clear

Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-GSK3b (S9) in MCF7 cells

Data show that treatment of MCF7 cells with Insulin stimulates phosphorylation of GSK3β at S9.

Inhibition of Phospho-GSK3b (S9) in MCF7 cells

Data show that treatment of MCF7 cells with UCN-01 inhibits phosphorylation of GSK3β at S9 by Insulin.

The Z’-factor value was determined using a total of 48 wells for each treatment group. The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Phospho-GSK3β (S9) TR-FRET Cell Signaling Assay Kit

Additional information

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Phospho-GSK3β (S9) TR-FRET Cell Signaling Assay Kit

Additional information

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TOOLBOX REAGENTS