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THUNDER™ Phospho-eIF2α (S51) TR-FRET Cell Signaling Assay Kit

2019-12-12

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EIF2α (S51) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: KIT-EIF2aP-100 Category:

Components
Eu-phospho-EIF2α (5 µL)
Acc-phospho-EIF2α (20 µL)
EIF2α control lysate (100 µL)
5X Lysis Buffer 1 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human

USD$409.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-eIF2α (S51) in Hela cells
Data show that treatment of HeLa cells with Calyculin A stimulates phosphorylation of eIF2α at S51.
Z'-factor determination in Hela cells
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
Hela Control cell lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.