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THUNDER™ Phospho-BAD (S112) TR-FRET Cell Signaling Assay Kit

2019-12-12

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-BAD (S112) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: KIT-BADP-100 Category:

Components
Eu-phospho-BAD (5 µL)
Acc-phospho-BAD (20 µL)
BAD control lysate (100 µL)
5X Lysis Buffer 1 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human

USD$409.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-BAD (S112) in MCF7 cells
Data show that treatment of MCF7 cells with PMA stimulates phosphorylation of BAD at S112
Inhibition of Phospho-BAD (S112) in MCF7 cells
Data show that treatment of MCF7 cells with UCN-01 inhibits phosphorylation of BAD at S112 by PMA
Z'-factor determination in MCF7 cells
The Z’-factor value of 0.76 indicates that the assay is robust and suitable for HTS
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values