Products menu

THUNDER™ Total JNK1/2/3 TR-FRET Cell Signaling Assay Kit

2019-12-13

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total JNK1/2/3 in cell lysates. This kit can be used to normalize data obtained with the Phospho-JNK1/2/3 (T183/Y185) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: N/A Category:

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-JNK1-2-3 (T183-Y185) in HEK293 cells
Data show that treatment of HEK293 cells with Anisomycin stimulates phosphorylation of JNK1/2/3 at T183/Y185 but does not affect the levels of total JNK1/2/3.
Inhibition of Phospho-JNK1-2-3 (T183-Y185) in HEK293 cells
Data show that treatment of HEK293 cells with BX 795 inhibits phosphorylation of JNK1/2/3 at T183/Y185 by Anisomycin, but does not affect the levels of total JNK1/2/3.
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.