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THUNDER™ Total CREB TR-FRET Cell Signaling Assay Kit

2019-12-12

View technical data sheet

USD$409.00 – USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total CREB in cell lysates. This kit can be used to normalize data obtained with the Phospho-CREB (S133) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: N/A Category: CREB

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Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-CREB (S133) in NIH3T3 cells

Data show that treatment of NIH3T3 cells with Forskolin stimulates phosphorylation of CREB at S133 but does not affect the levels of total CREB.

Inhibition of Phospho-CREB (S133) in A431 cells

Data show that treatment of A431 cells with Erlotinib inhibits phosphorylation of CREB at S133 by EGF, but does not affect the levels of total CREB.

B lymphocyte contro lysate titration

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

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