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THUNDER™ TR-FRET Acceptor-labeled Anti-6xHis

2022-02-10

USD$192.00USD$1,382.00

Purified mouse monoclonal anti-6x-Histidine tag antibody labeled with the THUNDER™ far-red (FR) acceptor fluorophore. The antibody detects His tags localized at the amino- or carboxyl‑terminus and is therefore suitable for capturing His-tagged proteins in THUNDER™ TR-FRET assays. A detailed assay protocol and full assay validation can be found in the Technical Data Sheet.

Note: His-tag® is a registered trademark of EMD Biosciences.

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FR-anti-6xHis validation
Titration of biotinylated 6xHis using Eu-labeled streptavidin, FR-labeled anti-6xHis and THUNDER 1X TR-FRET Detection Buffer. The plate was read on an EnVision® plate reader (flash lamp excitation) after 1 hour of incubation at room temperature.