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THUNDER™ Total RIPK1 TR-FRET Cell Signaling Assay Kit

2024-07-17

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total RIPK1 (both phosphorylated and non-phosphorylated RIPK1) in cell lysates. This kit can be used to normalize data obtained with the Phospho-RIPK1 (S166) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Inhibition of Phospho-RIPK1 in HT-29 cells
Data show that treatment of HT-29 cells with Necrostatin-1S inhibits phosphorylation of RIPK1 at S166 by TNF-alpha (in the presence of SM-164 and Z-VAD) but does not have a major effect on the levels of total RIPK1.
HT-29 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.