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THUNDER™ Total BRD4 TR-FRET Cell Signaling Assay Kit

2025-04-17

USD$430.00USD$8,809.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Human Total BRD4 (both phosphorylated and non-phosphorylated) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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Inhibition of Total BRD4 in MCF7 cells
Data show that treatment of MCF7 cells with the PROTAC compound dBET6 degrades total BRD4 in a concentration-dependent manner.
Inhibition of Total BRD4 in MCF7 cells
Data show that treatments of MCF7 cells with different PROTAC compounds degrade total BRD4. In contrast, BRD4 remains stable following treatment with an irrelevant compound.