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THUNDER™ Phospho-TBK1 (S172) TR-FRET Cell Signaling Assay Kit

2021-06-25

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-TBK1/NAK (S172) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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Data show that treatment of MCF7 cells with Calyculin A stimulates phosphorylation of TBK1/NAK at S172.
Data show that treatment of MCF7 cells with BX-795 inhibits phosphorylation of TBK1/NAK at S172 by Calyculin A.
The Z’-factor value of 0.70 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
Data show the robustness of the assay following overnight incubation.