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THUNDER® Phospho-SMAD3 (S423/S425) TR-FRET Cell Signaling Assay Kit

2025-06-18

Price range: USD$409.00 through USD$8,390.00

Sandwich TR-FRET immunoassay designed for the semi-quantitative detection of Phospho-SMAD3 (S423/S425) in cell lysates. The assay uses the advanced THUNDER® TR-FRET technology and a matched antibody pair specifically optimized and validated for TR-FRET. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. The assay is compatible with both adherent and suspension cells, and can be run under either a one-plate or two-plate protocol. Both methods are “no-wash”.

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with TGFβ1 stimulates phosphorylation of SMAD3 at S423/S425.
Inhibition of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with SB 431542 inhibits phosphorylation of SMAD3 at S423/S425 by TGFβ1.
Z'-factor determination in A549 cells
The Z’-factor value of 0.89 indicates that the assay is robust and suitable for HTS.
A549 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

New clients get a 40% discount and clients get a 25% discount until 27/2/2026

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