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THUNDER™ Phospho-SMAD3 (S423/S425) TR-FRET Cell Signaling Assay Kit

2025-06-18

Price range: USD$409.00 through USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SMAD3 (S423/S425) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with TGFβ1 stimulates phosphorylation of SMAD3 at S423/S425.
Inhibition of Phospho-SMAD3 (S423/S425) in A549 cells
Data show that treatment of A549 cells with SB 431542 inhibits phosphorylation of SMAD3 at S423/S425 by TGFβ1.
Z'-factor determination in A549 cells
The Z’-factor value of 0.89 indicates that the assay is robust and suitable for HTS.
A549 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.