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THUNDER™ Phospho-RIPK1 (S166) + Total RIPK1 TR-FRET Cell Signaling Assay Kit

2024-07-18

$1,375.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-RIPK1 (S166) and Total RIPK1 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total RIPK1 proteins in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Inhibition of Phospho-RIPK1 (S166) in HT-29 cells
Data show that treatment of HT-29 cells with Necrostatin-1S inhibits phosphorylation of RIPK1 at S166 by TNF-alpha (in the presence of SM-164 and Z-VAD) but does not have a major effect on the levels of total RIPK1.
Phospho-RIPK1-Z'-factor in HT-29
The Z’-factor value of 0.74 indicates that the Phospho-RIPK1 assay is robust and suitable for HTS.
HT-29 control lysate titration (QC Test) - Phospho-RIPK1
Data show a linear relationship between lysate dilutions and TR-FRET ratio values following 1/2 dilution.
HT-29 control lysate titration (QC Test) - Total RIPK1
Data show a linear relationship between lysate dilutions and TR-FRET ratio values following 1/2 dilution.