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THUNDER™ Phospho-Rb (S807/S811) + Total Rb TR-FRET Cell Signaling Assay Kit

2025-01-20

USD$1,375.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Rb (S807/S811) and Total Rb in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total Rb proteins in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: KIT-RBS807PT-500 Categories: ,

400 points for phospho; 100 points for total

Components
Eu-phospho-Rb S807 (20 µL)
Acc-phospho-Rb S807 (80 µL)
Eu-Total Rb (5 µL)
Acc-Total Rb (20 µL)
Rb control lysate (200 µL)
5X Lysis Buffer 3 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human

USD$1,375.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Inhibition of Phospho-Rb (S807/S811) in HT-29 cells
Data show that treatment of HT-29 cells with palbociclib inhibits both phospho-Rb (S807/S811) and total Rb. However, levels of housekeeping total GAPDH (measured as a control) remain stable.
HT-29 control lysate titration (QC Test) - Phospho-Rb (S807/S811)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
HT-29 control lysate titration (QC Test) - Total Rb
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.