Sandwich TR-FRET immunoassay for the quantitative measurement of human VEGF165 (VEGF; VEGFA; VPF) in cell culture supernatants. The kit does not include a recombinant human VEGF standard, which must be purchased separately from PeproTech® (catalogue number 100-20). A detailed assay protocol and full assay validation can be found in the Technical Data Sheet.
Key features and benefits:
Homogeneous: no wash, easy and rapid protocol
High sensitivity (15 µL sample), specificity, robustness, and reproducibility