Sandwich TR-FRET immunoassay for the quantitative measurement of human Pro-Collagen I alpha 1 (COL1A1; COLIA1) in cell culture supernatants. The kit does not include a recombinant human COL1A1 standard, which must be purchased separately from R&D Systems® (catalogue number 6220-CL). A detailed assay protocol and full assay validation can be found in the Technical Data Sheet.
Key features and benefits:
Homogeneous: no wash, easy and rapid protocol
High sensitivity (15 µL sample), specificity, robustness, and reproducibility
