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THUNDER™ High Performance Total STAT6 TR-FRET Cell Signaling Assay Kit

2024-07-18

$430.00$8,809.00

High performance sandwich TR-FRET immunoassay for semi-quantitative detection of Human Total STAT6 (both phosphorylated and non-phosphorylated) in cell lysates. This kit can be used to normalize data obtained with the Phospho-STAT6 (Y641) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: This kit is a new and improved high performance version of catalog number KIT-STAT6T-XXX, which will remain available for purchase.

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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Stimulation of Phospho-STAT6 (Y641) in HeLa cells
Data show that treatment of HeLa cells with IL-4 stimulates phosphorylation of STAT6 at Y641, but does not affect the levels of total STAT6.
Total SAT6 (Y641) in HeLa cells-Time course of detection
Data show that robust S/B ratio and pharmacology can be obtained after 1h of incubation.
Inhibition of Phospho-STAT6 (Y641) in HeLa cells
Data show that treatment of HeLa cells with JAK Inhibitor 1 inhibits phosphorylation of STAT6 at Y641 by IL-4, but does not affect the levels of total STAT6.
HeLa control lysate titration (QC test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.