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THUNDER™ High Performance Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit

2024-07-06

USD$430.00USD$8,809.00

High performance sandwich TR-FRET immunoassay for semi-quantitative detection of Human and Mouse Phospho-STAT3 (Y705) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER

Note: This kit is a new and improved high performance version of catalog number KIT-STAT3P-XXX, which will remain available for purchase. See the new Application Note for a comparison of THUNDER® High Performance Phospho-STAT3 versus HTRF® and AlphaLISA SureFire® Ultra. The High-Performance THUNDER assay outperformed HTRF and matched SureFire in terms of assay sensitivity while being simpler, faster, and more cost-effective.

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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High Performance versus Standard Phospho-STAT3 Assay Kit
Data show the superior S/B ratio obtained with the High Performance Phospho-STAT3 (Y705) Assay compared to the standard version of the assay
Stimulation of Phospho-STAT3 (Y705) in HeLa cells
Data show that treatment of HeLa cells with IFNα2b stimulates phosphorylation of STAT3 at Y705. Robust S/B ratio and pharmacology are obtained after 1 h of incubation.
Inhibition of Phospho-STAT3 (Y705) in HeLa cells
Data show that treatment of HeLa cells with JAK inhibitor 1 inhibits phosphorylation of STAT3 at Y705 by IFNα2b. Robust S/B ratio and pharmacology are obtained after 1 h of incubation.
Z'-factor determination in HeLa cells
The Z’-factor value of 0.94 indicates that the assay is robust and suitable for HTS.
Phospho-STAT3 benchmarking-HTRF
The THUNDER assay outperforms the HTRF assay in terms of S/B ratio.
Phospho-STAT3 benchmarking-SureFire
The THUNDER assay generates the same S/B ratio as the SureFire assay but THUNDER takes only 1 hour.
Stimulation of Phospho-STAT3 (Y705) in NIH/3T3 cells
Data show that the High Performance kit detects mouse Phospho-STAT3 (Y705).