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THUNDER™ Extreme Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit

2021-06-23

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of low levels of Phospho-ERK1/2 (T202/Y204) in cell lysates. This high-sensitivity kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER

SKU: KIT-XTM-ERKP-100 Categories: ,

Components
Eu-phospho-XTMERK1/2 (5 µL)
Acc-phospho-XTMERK1/2 (20 µL)
ERK1/2 control lysate (100 µL)
5X Lysis Buffer 1 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human, Mouse

USD$409.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

STIMULATION OF PHOSPHO-ERK1/2 (T202/Y204) IN HEK293 CELLS
Data show that treatment of HEK293 cells with EGF stimulates phosphorylation of ERK1/2 at T202/Y204.
INHIBITION OF BASAL PHOSPHO-ERK1/2 (T202/Y204) IN H358 CELLS
Data show that treatment of H358 cells with RMC-4550 inhibits basal phosphorylation of ERK1/2 at T202/Y204.
INHIBITION OF BASAL PHOSPHO-ERK1/2 (T202/Y204) IN MCF7 CELLS
Data show that treatment of MCF7 cells with L779,450 inhibits basal phosphorylation of ERK1/2 at T202/Y204.
INHIBITION OF BASAL PHOSPHO-ERK1/2 (T202/Y204) IN B LYMPHOCYTES
Data show that treatment of B lymphocyte cells with L779,450 inhibits basal phosphorylation of ERK1/2 at T202/Y204.