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THUNDER™
TR-FRET

CELL SIGNALING ASSAY KITS

THUNDER™
TR-FRET

BIOMARKER ASSAY KITS
Human IFNβ
Mouse IFNβ
Human IFNα
Human IFNγ
Mouse IFNγ
Human IL-1α
Mouse IL-1α
Human IL-1β
Mouse IL-1β
Human IL-2
Mouse IL-2
Human IL-6
Mouse IL-6
Human IL-8/CXCL8
Human IL-10
Human IL-11
Human IL-12/IL-23 p40
Human IL-12 p70
Human IL-17A
Human IL-18
Human Fibronectin
Human Insulin
Human CCL2/MCP1
Mouse CCL2/MCP1
Human CCL3/MIP1α
Human CCL4/MIP-1β
Human CCL17/TARC
Human CXCL1/GROα
Human CXCL9/MIG
Human TNFα
Human MMP-3
Human MMP-7
Human PD-L1
Human TNFα
Mouse TNFα
Human GM-CSF
Human COL1A1
Human VEGF
Mouse VEGF
Human IgG (Fc)
Mouse IgG
Rabbit IgG (Fc)
CHO HCP

THUNDER™
TR-FRET

cAMP ASSAY KITS
cAMP assay kit

THUNDER™
TR-FRET
TOOLBOX REAGENTS

EUROPIUM LABELED
Europium-labeled anti-6xHis
Europium-labeled anti-FLAG
Europium-labeled anti-GST
Europium-labeled anti-V5
Europium-labeled anti-human IgG
Europium-labeled anti-mouse IgG
Europium-labeled anti-rabbit IgG
Europium-labeled streptavidin
ACCEPTOR LABELED
Acceptor-labeled anti-6xHis
Acceptor-labeled anti-FLAG
Acceptor-labeled anti-GST
Acceptor-labeled anti-V5
Acceptor-labeled anti-human IgG
Acceptor-labeled anti-mouse IgG
Acceptor-labeled anti-rabbit IgG
Acceptor-labeled streptavidin

COMPLEMENTARY PRODUCTS

READER CONTROL
THUNDER™ Reader Control TR-FRET Kit
POSITIVE CONTROL LYSATES
THUNDER™ 4EBP1 Control lysate
THUNDER™ AKT pan Control lysate
THUNDER™ BAD Control lysate
THUNDER™ CREB Control lysate
THUNDER™ EGFR Control lysate
THUNDER™ eIF2α Control lysate
THUNDER™ ERK Control lysate
THUNDER™ GAPDH Control lysate
THUNDER™ GSK3β Control lysate
THUNDER™ INR Control lysate
THUNDER™ JNK Control lysate
THUNDER™ MEK1 Control lysate
THUNDER™ MET Control lysate
THUNDER™ NF-κB Control lysate
THUNDER™ p38 Control lysate
THUNDER™ p53 Control lysate
THUNDER™ P70S6 Control lysate
THUNDER™ RPS6 Control lysate
THUNDER™ SLP-76 Control lysate
THUNDER™ SMAD2 Control lysate
THUNDER™ SRC Control lysate
THUNDER™ STAT3 Control lysate
THUNDER™ STAT5 Control lysate
THUNDER™ STAT6 Control lysate
LYSIS BUFFERS
THUNDER™ Lysis Buffer 1 (5X)
THUNDER™ Lysis Buffer 2 (5X)
THUNDER™ Lysis Buffer 3 (5X)
THUNDER™ Lysis Buffer 4 (5X)
THUNDER™ Lysis Buffer 5 (5X)
THUNDER™ TR-FRET cAMP Detection Buffer (5X)
DETECTION BUFFER
THUNDER™ TR-FRET Assay Buffer 1 (5X)
THUNDER™ TR-FRET Assay Buffer 2 (5X)
THUNDER™ TR-FRET Assay Buffer 3 (5X)
THUNDER™ TR-FRET Assay Buffer 4 (5X)
THUNDER™ TR-FRET cAMP Detection Buffer (5X)
THUNDER™ TR-FRET Detection Buffer (10X)
THUNDER™ TR-FRET Kinase Assay Buffer A (5X)
THUNDER™ TR-FRET Kinase Assay Buffer B (5X)
THUNDER™ TR-FRET PPI Assay Buffer 1 (5X)
THUNDER™ TR-FRET PPI Assay Buffer 2 (5X)
THUNDER™ TR-FRET PPI Assay Buffer 3 (5X)
THUNDER™ TR-FRET PPI Assay Buffer 4 (5X)
PHOSPHATASE INHIBITOR COCKTAIL
THUNDER™ Phosphatase Inhibitor Cocktail (100X)

THUNDER™ Phospho-SLP-76 (S376) TR-FRET Cell Signaling Assay Kit

2019-12-13
COMPLEMENTARY REAGENTS THUNDER™ SLP-76 Control lysate THUNDER™ TR-FRET Detection Buffer (10X) THUNDER™ Lysis Buffer 2 (5X) THUNDER™ Phosphatase Inhibitor Cocktail (100X)
View technical data sheet View generic user manual

USD$409.00 – USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SLP-76 (S376) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: the Phospho-SLP-76 (S376) Assay Kit uses a new antibody pair that significantly improves the assay performance. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay performs comparably to an alternative TR-FRET assay available.

SKU: N/A Category:

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Related products

Benchmarking of THUNDER Phospho-SLP-76 (S376) vs Company A
The THUNDER assay shows a higher S/B ratio than Company A assay
Benchmarking of THUNDER Phospho-SLP-76 (S376) vs Company B
The THUNDER and Company B assays show comparable S/B ratios
Stimulation of Phospho-SLP-76 (S376) in Jurkat cells
Data show that treatment of Jurkat cells with H2O2 stimulates phosphorylation of SLP-76 at S376
Z'-factor determination in Jurkat cells
The Z’-factor value of 0.92 indicates that the assay is robust and suitable for HTS
Jurkat control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
All-in-one-well Phopsho-SLP-76 (S376) assay
Data show that stimulation of phospho-SLP-76 (S376) by H2O2 in Jurkat cells can be detected using the 1-plate assay protocol in both LV 384 and HA 96-well plates
Bioauxilium specializes in the design, development and manufacturing of ready-to-use TR-FRET assay kits that simplify laboratory workflow and accelerate drug discovery and life sciences research.
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