Name: THUNDER™ Phospho-SLP-76 (S376) TR-FRET Cell Signaling Assay Kit
SKU: 326
Availability: InStock

THUNDER™ Phospho-SLP-76 (S376) TR-FRET Cell Signaling Assay Kit

2019-12-13

View technical data sheet View generic user manual

USD$409.00 – USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SLP-76 (S376) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: the Phospho-SLP-76 (S376) Assay Kit uses a new antibody pair that significantly improves the assay performance. Assay benchmarking is shown in the updated Application Note. The THUNDER® assay outperforms HTRF® and matches LANCE® Ultra.

SKU: KIT-SLP76P-100 Category: SLP-76

Size

Clear

Components
Eu-phospho-SLP76 (5 µL)
Acc-phospho-SLP76 (20 µL)
SLP76 control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human

USD$409.00

Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

Benchmarking of THUNDER Phospho-SLP-76 (S376) vs Company A

The THUNDER assay shows a higher S/B ratio than Company A assay

Benchmarking of THUNDER Phospho-SLP-76 (S376) vs Company B

The THUNDER and Company B assays show comparable S/B ratios

Stimulation of Phospho-SLP-76 (S376) in Jurkat cells

Data show that treatment of Jurkat cells with H2O2 stimulates phosphorylation of SLP-76 at S376

The Z’-factor value of 0.92 indicates that the assay is robust and suitable for HTS

Jurkat control lysate titration (QC Test)

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

All-in-one-well Phopsho-SLP-76 (S376) assay

Data show that stimulation of phospho-SLP-76 (S376) by H2O2 in Jurkat cells can be detected using the 1-plate assay protocol in both LV 384 and HA 96-well plates

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Phospho-SLP-76 (S376) TR-FRET Cell Signaling Assay Kit

Additional information

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Phospho-SLP-76 (S376) TR-FRET Cell Signaling Assay Kit

Additional information

Related products

THUNDER™ Total SLP-76 TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-SLP-76 (S376) + Total SLP-76 TR-FRET Cell Signaling Assay Kit

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS