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THUNDER™ Phospho-p53 (S15) + Total p53 TR-FRET Cell Signaling Assay Kit

2019-12-13

USD$1,375.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p53 (S15) and Total p53 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total p53 proteins in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: KIT-P53PT-500 Category:

400 points for phospho; 100 points for total

Components
Eu-phospho-p53 (20 µL)
Acc-phospho-p53 (80 µL)
Eu-Total p53 (5 µL)
Acc-Total p53 (20 µL)
p53 control lysate (200 µL)
5X Lysis Buffer 2 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)

Reactivity
Human

USD$1,375.00
Stimulation of Phospho-P53 (S15) in HEK293 cells
Data show that treatment of HEK293 cells with Etoposide stimulates phosphorylation of p53 at Ser15, but does not affect the levels of total p53
HEK293 control lysate titration (QC Test) Phospho-P53(S15)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
HEK293 control lysate titration (QC Test) Total P53
Data show a linear relationship between lysate dilutions and TR-FRET ratio values