Name: THUNDER™ Phospho-MEK1 (S218/S222) TR-FRET Cell Signaling Assay Kit
SKU: 266
Availability: InStock

THUNDER™ Phospho-MEK1 (S218/S222) TR-FRET Cell Signaling Assay Kit

2019-12-13

View technical data sheet View generic user manual

USD$409.00 – USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-MEK1 (S218/S222) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: N/A Category: MEK1

Size

Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-MEK1 (S218-S222) in HeLa cells

Data show that treatment of HeLa cells with PMA stimulates phosphorylation of MEK1 at S218/S222

Inhibition of Phospho-MEK1 (S218-S222) in HeLa cells

Data show that treatment of HeLa cells with L779,450 inhibits phosphorylation of MEK1 at S218/S222 by PMA.

The Z’-factor value of 0.76 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

Data show that stimulation of phospho-MEK1 (S218/S222) in HeLa cells can be detected using the 1-plate assay protocol.

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Phospho-MEK1 (S218/S222) TR-FRET Cell Signaling Assay Kit

Additional information

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Phospho-MEK1 (S218/S222) TR-FRET Cell Signaling Assay Kit

Additional information

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