Sandwich TR-FRET immunoassay for the quantitative measurement of human CCL2 (MCP-1; MCAF; JE) in cell culture supernatants. The kit does not include a recombinant human CCL2 standard, which must be purchased separately from R&D Systems® (catalog number 279-MC-010). A detailed assay protocol and full assay validation can be found in the Technical Data Sheet.
Key features and benefits:
Homogeneous: no wash, easy and rapid protocol
High sensitivity (15 µL sample), specificity, robustness, and reproducibility
Standard curve for the human CCL2/MCP1 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using lamp excitation.
Standard curve for the human CCL2/MCP1 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using laser excitation.
Assay performance on three different microplate readers.
Supernatants from PBMC cells treated for two days with LPS and IL-2 were serially diluted with culture medium and assayed for CCL-2. Recovery values between 94-114% of the 4-fold diluted sample demonstrate assay parallelism.
