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THUNDER™ Phospho-ERK1/2 (T202/Y204) + Total ERK1/2 TR-FRET Cell Signaling Assay Kit

2019-12-13

USD$1,375.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-ERK1/2 (T202/Y204) and Total ERK1/2 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total ERK1/2 proteins in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: KIT-ERKPT-500 Category:

400 points for phospho; 100 points for total

Components
Eu-phospho-ERK (20 µL)
Acc-phospho-ERK (80 µL)
Eu-Total ERK (5 µL)
Acc-Total ERK (20 µL)
ERK control lysate (200 µL)
5X Lysis Buffer 1 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)

Reactivity
Human, Mouse

USD$1,375.00
Stimulation of Phospho-ERK1-2 (T202-Y204) in HEK293 cells
Data show that treatment of HEK293 cells with EGF stimulates phosphorylation of ERK1/2 at T202/Y204, but does not affect the levels of Total ERK1/2.
Inhibition of Phospho-ERK1-2 (T202-Y204) in HEK293 cells
Data show that treatment of HEK293 cells with Erlotinib inhibits phosphorylation of ERK1/2 at T202/Y204 by EGF, but does not affect the levels of Total ERK1/2.
HEK293 control lysate titration (QC Test) Phospho-ERK1-2 (T202-Y204)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
HEK293 control lysate titration (QC Test) Total-ERK1-2)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.