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THUNDER™ Total GAPDH TR-FRET Cell Signaling Assay Kit

2019-12-13

Price range: USD$409.00 through USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total GAPDH in cell lysates. Measurement of GAPDH may be a useful internal control for monitoring total protein levels when measuring changes in specific phosphorylated and/or total proteins. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: owing to the high sensitivity of this kit, the lysates need to be diluted 10-fold in Lysis Buffer 3 or 4 before testing. This kit is only compatible with cell lysates generated with the Lysis Buffer 3 or Lysis Buffer 4.

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Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Total GAPDH Adherent cells
Detection of total GAPDG in different adherent cell lines.
Total GAPDH Suspension cells
Detection of total GAPDG in different suspension cell lines.
Detection GAPDH by Western-blot
A specific band was detected for GAPDH at approximately 37 kDa.
Total GAPDH versus Total Rb
Data show that treatment of HT-29 cells with palbociclib (a Cdk4 and Cdk 6 inhibitor) inhibits both total Rb and phospho-Rb (S780). In contrast, levels of housekeeping total GAPDH (measured as a control) remain stable.
Total GAPDH versus Total IRAK4
Data show that treatment of THP-1 cells with the PROTAC compound KT-474 degrades total IRAK4. In contrast, levels of housekeeping total GAPDH (measured as a control) remain stable.

New clients get a 40% discount and clients get a 25% discount until 31/1/2026

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