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THUNDER™ Phospho BTK (Y223) + Total BTK TR-FRET Cell Signaling Assay Kit

2021-06-23

USD$1,375.00

Sandwich TR-FRET immunoassays for semi-quantitative detection of Phospho-BTK (Y223) and Total BTK in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total BTK proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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100 points, 500 points, 2500 points, 5000 points, 10000 points

Total BTK in Raji cells-stimulation
Data show that treatment of Raji cells with pervanadate stimulates phosphorylation of BTK at Y223 but does not affect the levels of total BTK.
Total BTK in Raji cells-inhibition
Data show that treatment of Raji cells with ibrutinib inhibits phosphorylation of BTK at Y223 by pervanadate, but does not affect the levels of total BTK.
Phospho-BTK-QC test
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
Total BTK-QC test
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.