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THUNDER™ Phospho-Rb (S780) TR-FRET Cell Signaling Assay Kit

2024-10-29

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Rb (S780) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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Inhibition of Phospho-Rb-S780 in HT-29 cells
Data show that treatment of HT-29 cells with palbociclib inhibits phosphorylation of Rb at S780.
Z'-factor of Phospho-Rb (S780) in HT-29 cells
The Z’-factor value of 0.72 indicates that the assay is robust and suitable for HTS.
HT-29 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.