Products menu

THUNDER™ Phospho-LCK (Y394) TR-FRET Cell Signaling Assay Kit

2021-06-25

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-LCK (Y394) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: N/A Categories: ,

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Data show that treatment of Jurkat cells with Pervanadate stimulates phosphorylation of LCK at Y394.
The Z’-factor value of 0.72 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
Phospho-LCK benchmarking
Lysates were generated with the corresponding kit's Lysis Buffer. Data show that the THUNDER Assay Kit generates superior S/B ratios compared to the Competitor's assay.