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THUNDER™ Phospho-BTK (Y223) TR-FRET Cell Signaling Assay Kit

2021-06-23

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-BTK (Y223) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: KIT-BTKP-100 Categories: ,

Components
Eu-phospho-BTK (5 µL)
Acc-phospho-BTK (20 µL)
BTK control lysate (500 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human

USD$409.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-BTK (Y223) in Raji cells
Data show that treatment of Raji cells with Pervanadate stimulates phosphorylation of BTK at Y223
Inhibition of Phospho-BTK (Y223) in Raji cells
Data show that treatment of Raji cells with Ibrutinib inhibits phosphorylation of BTK at Y223 by Pervanadate
Z'-factor determination in Raji cells
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS
Phospho-BTK (Y223) Control lysate titration
Data show a linear relationship between lysate dilutions and TR-FRET ratio values