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THUNDER™ Phospho-STAT3 (Y705) + Total STAT3 TR-FRET Cell Signaling Assay Kit

2019-12-13

USD$1,375.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT3 (Y705) and Total STAT3 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total STAT3 proteins in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: the Phospho-STAT3 (Y705) + Total STAT3 Assay Kit uses two new antibody pairs that significantly improve the assay performance (see the updated Application Note).

Product citation: PubMed 34570089

SKU: KIT-STAT3PT-500 Category:

400 points for phospho; 100 points for total

Components
Eu-phospho-STAT3 (20 µL)
Acc-phospho-STAT3 (80 µL)
Eu-Total STAT3 (5 µL)
Acc-Total STAT3 (20 µL)
STAT3 control lysate (200 µL)
5X Lysis Buffer 2 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)

Reactivity
Human, Mouse

USD$1,375.00
Stimulation of Phospho-STAT3 (Y705) in HeLa cells
Data show that treatment of HeLa cells with IFNα stimulates phosphorylation of STAT3 (Y705), but does not affect the levels of total STAT3
Inhibition of Phospho-STAT3 (Y705) in HeLa cells
Data show that treatment of HeLa cells with JAK Innibitor 1 inhibits phosphorylation of STAT3 (Y705) by IFNα, but does not affect the levels of total STAT3
HeLa control lysate titration (QC Test) Phospo-STAT3 (Y705)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
HeLa control lysate titration (QC Test) Total STAT3
Data show a linear relationship between lysate dilutions and TR-FRET ratio values