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THUNDER™ Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit

2019-12-13

View technical data sheet View product brochure View generic user manual

USD$409.00 – USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT3 (Y705) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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Note: the Phospho-STAT3 (Y705) Assay Kit uses a new antibody pair that significantly improves the assay performance. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay outperforms the two other TR-FRET assays available.

Product citation: PubMed 34570089

SKU: N/A Category: STAT3

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Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

New versus Old Phospho-STAT3 Assay Kit

Data show the superior performance of the new Phospho-STAT3 (Y705) Assay compared to the previous version of the assay

Stimulation of Phospho-STAT3 (Y705) in HeLa cells

Data show that treatment of HeLa cells with IFNα stimulates phosphorylation of STAT3 at Y705

Inhibition of Phospho-STAT3 (Y705) in HeLa cells

Data show that treatment of HeLa cells with JAK Innibitor 1 inhibits phosphorylation of STAT3 at Y705 by IFNα

Z'-factor determination in HeLa cells

The Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS

HeLa control lysate titration (QC Test)

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

Phospho-STAT3 benchmarking-THUNDER

The THUNDER assay shows the highest S/B ratios between 1 and 4 hours of incubation

Phospho-STAT3 benchmarking-Company A

Company A assay shows a comparable S/B ratio to the THUNDER assay only after overnight incubation

Phospho-STAT3 benchmarking-Company B

Company B assay shows the lowest S/B ratios at all incubation times

Phospho-STAT3 stimulation in NIH/3T3 cells

Data show that treatment of NIH/3T3 cells with reference cytokines stimulates phosphorylation of STAT3 at Y705.

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