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THUNDER™ Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit

2019-12-13

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT3 (Y705) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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Note: the Phospho-STAT3 (Y705) Assay Kit uses a new antibody pair that significantly improves the assay performance. Assay benchmarking of THUNDER® Phospho-STAT3 against HTRF® and LANCE® Ultra is shown in the updated Application Note. The THUNDER® assay outperforms the two other TR-FRET assays available (HTRF® and LANCE® Ultra).

Note: a new and improved version of this kit is available: High Performance Phospho-STAT3 (Y705) Assay Kit

Product citation: PubMed 34570089

SKU: KIT-STAT3P-100 Category:

Components
Eu-phospho-STAT3 (5 µL)
Acc-phospho-STAT3 (20 µL)
STAT3 control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human, Mouse

USD$409.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

New versus Old Phospho-STAT3 Assay Kit
Data show the superior performance of the new Phospho-STAT3 (Y705) Assay compared to the previous version of the assay
Stimulation of Phospho-STAT3 (Y705) in HeLa cells
Data show that treatment of HeLa cells with IFNα stimulates phosphorylation of STAT3 at Y705
Inhibition of Phospho-STAT3 (Y705) in HeLa cells
Data show that treatment of HeLa cells with JAK Innibitor 1 inhibits phosphorylation of STAT3 at Y705 by IFNα
Z'-factor determination in HeLa cells
The Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS
HeLa control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values
Phospho-STAT3 benchmarking-THUNDER
The THUNDER assay shows the highest S/B ratios between 1 and 4 hours of incubation
Phospho-STAT3 benchmarking-Company A
Company A (HTRF) assay shows a comparable S/B ratio to the THUNDER assay only after overnight incubation
Phospho-STAT3 benchmarking-Company B
Company B (LANCE Ultra) assay shows the lowest S/B ratios at all incubation times
Phospho-STAT3 stimulation in NIH/3T3 cells
Data show that treatment of NIH/3T3 cells with reference cytokines stimulates phosphorylation of STAT3 at Y705.