Name: THUNDER™ Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit
SKU: 341
Availability: InStock

THUNDER™ Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit

2019-12-13

View technical data sheet View product brochure View generic user manual

USD$409.00 – USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT3 (Y705) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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Note: the Phospho-STAT3 (Y705) Assay Kit uses a new antibody pair that significantly improves the assay performance. Assay benchmarking of THUNDER® Phospho-STAT3 against HTRF® and LANCE® Ultra is shown in the updated Application Note. The THUNDER® assay outperforms the two other TR-FRET assays available (HTRF® and LANCE® Ultra).

Note: a new and improved version of this kit is available: High Performance Phospho-STAT3 (Y705) Assay Kit

Product citation: PubMed 34570089

SKU: KIT-STAT3P-100 Category: STAT3

Size

Clear

Components
Eu-phospho-STAT3 (5 µL)
Acc-phospho-STAT3 (20 µL)
STAT3 control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human, Mouse

USD$409.00

Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

Data show the superior performance of the new Phospho-STAT3 (Y705) Assay compared to the previous version of the assay

Stimulation of Phospho-STAT3 (Y705) in HeLa cells

Data show that treatment of HeLa cells with IFNα stimulates phosphorylation of STAT3 at Y705

Inhibition of Phospho-STAT3 (Y705) in HeLa cells

Data show that treatment of HeLa cells with JAK Innibitor 1 inhibits phosphorylation of STAT3 at Y705 by IFNα

The Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

The THUNDER assay shows the highest S/B ratios between 1 and 4 hours of incubation

Company A (HTRF) assay shows a comparable S/B ratio to the THUNDER assay only after overnight incubation

Company B (LANCE Ultra) assay shows the lowest S/B ratios at all incubation times

Phospho-STAT3 stimulation in NIH/3T3 cells

Data show that treatment of NIH/3T3 cells with reference cytokines stimulates phosphorylation of STAT3 at Y705.

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit

Additional information

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit

Additional information

Related products

THUNDER™ Phospho-STAT3 (Y705) + Total STAT3 TR-FRET Cell Signaling Assay Kit

THUNDER™ Total STAT3 TR-FRET Cell Signaling Assay Kit

THUNDER™ High Performance Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS