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THUNDER™ Phospho-SMAD2 (S465/S467) TR-FRET Cell Signaling Assay Kit

2019-12-13

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SMAD2 (S465/S467) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Product Citation: PubMed 35664796

 

SKU: KIT-SMAD2P-100 Category:

Components
Eu-phospho-SMAD2 (5 µL)
Acc-phospho-SMAD2 (20 µL)
SMAD2 control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human

USD$409.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-SMAD2 (S465/S467) in HeLa cells
Data show that treatment of HeLa cells with TGFβ stimulates phosphorylation of SMAD2 at S465/S467
Inhibition of Phospho-SMAD2 (S465/S467) in HeLa cells
Data show that treatment of HeLa cells with SB 431542 inhibits phosphorylation of SMAD2 at S465/S467 by TGFβ
Z'-factor determination in HeLa cells
The Z’-factor value of 0.65 indicates that the assay is robust and suitable for HTS
HeLa control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values