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THUNDER™ Phospho-SMAD2 (S465/S467) TR-FRET Cell Signaling Assay Kit

2019-12-13

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-SMAD2 (S465/S467) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Product Citation: PubMed 35664796

 

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Stimulation of Phospho-SMAD2 (S465/S467) in HeLa cells
Data show that treatment of HeLa cells with TGFβ stimulates phosphorylation of SMAD2 at S465/S467
Inhibition of Phospho-SMAD2 (S465/S467) in HeLa cells
Data show that treatment of HeLa cells with SB 431542 inhibits phosphorylation of SMAD2 at S465/S467 by TGFβ
Z'-factor determination in HeLa cells
The Z’-factor value of 0.65 indicates that the assay is robust and suitable for HTS
HeLa control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values