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THUNDER™ Phospho-p53 (S15) TR-FRET Cell Signaling Assay Kit

2019-12-13

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p53 (S15) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: KIT-P53P-100 Category:

Components
Eu-phospho-p53 (5 µL)
Acc-phospho-p53 (20 µL)
p53 control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human

USD$409.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-P53 (S15) in HEK293 cells
Data show that treatment of HEK293 cells with Etoposide stimulates phosphorylation of p53 at Ser15
Z'-factor determination in HEK293 cells
The Z’-factor value of 0.6 indicates that the assay is robust and suitable for HTS
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TRFRET ratio values