Products menu

THUNDER™ Phospho-NF-kB (S536) TR-FRET Cell Signaling Assay Kit

2019-12-13

$409.00$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-NF-κB (S536) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Product citation: PubMed 36129967

SKU: N/A Category:

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-NF-kB (S536) in HeLa cells
Data show that treatment of HeLa cells with TNFα stimulates phosphorylation of NF-kB at S536
Stimulation of Phospho-NF-kB (S536) in HeLa cells
Data show that treatment of HeLa cells with IKK-16 inhibits phosphorylation of NF-kB at S536 by TNFα
Z'-factor determination in HeLa cells
The Z’-factor value of 0.78 indicates that the assay is robust and suitable for HTS
HeLa control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values