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THUNDER™ Phospho-NF-kB (S536) TR-FRET Cell Signaling Assay Kit

2019-12-13

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-NF-κB (S536) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Product citation: PubMed 36129967

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Stimulation of Phospho-NF-kB (S536) in HeLa cells
Data show that treatment of HeLa cells with TNFα stimulates phosphorylation of NF-kB at S536
Stimulation of Phospho-NF-kB (S536) in HeLa cells
Data show that treatment of HeLa cells with IKK-16 inhibits phosphorylation of NF-kB at S536 by TNFα
Z'-factor determination in HeLa cells
The Z’-factor value of 0.78 indicates that the assay is robust and suitable for HTS
HeLa control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values