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THUNDER™ Phospho-JNK1/2/3 (T183/Y185) TR-FRET Cell Signaling Assay Kit

2019-12-13

USD$409.00USD$8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-JNK1/2/3 (T183/Y185) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: KIT-JNKP-100 Category:

Components
Eu-phospho-JNK (5 µL)
Acc-phospho-JNK (20 µL)
JNK control lysate (100 µL)
5X Lysis Buffer 2 (1 mL)
10X TR-FRET Detection buffer (50 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)

Reactivity
Human

USD$409.00

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-JNK1-2-3 (T183-Y185) in HEK293 cells
Data show that treatment of HEK293 cells with Anisomycin stimulates phosphorylation of JNK1/2/3 at T183/Y185.
Inhibition of Phospho-JNK1-2-3 (T183-Y185) HEK293 cells
Data show that treatment of HEK293 cells with BX 795 inhibits phosphorylation of JNK1/2/3 at T183/Y185 by Anisomycin.
Z'-factor determination in HEK293 cells
The Z’-factor value of 0.82 indicates that the assay is robust and suitable for HTS.
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.