Sandwich TR-FRET immunoassay for the quantitative measurement of human IL-2 in cell culture supernatants. The kit does not include a recombinant human IL-2 standard, which must be purchased separately from R&D Systems® (catalogue number 202-IL). A detailed assay protocol and full assay validation can be found in the Technical Data Sheet. BEST SELLER
Key features and benefits:
Homogeneous: no wash, easy and rapid protocol
High sensitivity (15 µL sample), specificity, robustness, and reproducibility
Standard curve for the human IL-2 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using lamp excitation.
Standard curve for the human IL-2 TR-FRET assay run in a 384-well white plate and read on a TR-FRET enabled reader using laser excitation.
Supernatants from Jurkat cells treated overnight with PMA and anti-CD3 were serially diluted with culture medium and assayed for IL-2. Recovery values between 101-104% of the neat sample demonstrate assay parallelism.
