Products menu

THUNDER™ Total Rb TR-FRET Cell Signaling Assay Kit

2025-01-05

USD$430.00USD$8,809.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Human Total Rb (both phosphorylated and non-phosphorylated) in cell lysates. This kit can be used to normalize data obtained with the Phospho-Rb (S780) kit and the Phospho-Rb (S807/S811) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

SKU: N/A Categories: ,

Additional information

Size

100 points, 500 points, 2500 points, 5000 points, 10000 points

Inhibition of Total Rb in HT-29 cells
Data show that treatment of HT-29 cells with palbociclib inhibits both total Rb and phospho-Rb (S780). However, levels of housekeeping total GAPDH (measured as a control) remain stable.
Inhibition of Total Rb in HT-29 cells
Data show that treatment of HT-29 cells with palbociclib inhibits both total Rb and phospho-Rb (S807/S811). However, levels of housekeeping total GAPDH (measured as a control) remain stable.
HT-29 control lysate titration (QC test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.