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THUNDER™ Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit

2019-12-13

View technical data sheet View generic user manual

$409.00 – $8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-ERK1/2 (T202/Y204) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay outperforms the two other TR-FRET assays available. BEST SELLER

SKU: N/A Category: ERK1/2

Size

Clear

Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of PHOSPHO-ERK1-2 (T202-Y204) in HEK293 cells

Data show that treatment of HEK293 cells with EGF stimulates phosphorylation of ERK1/2 at T202/Y204.

Inhibition of Phospho-ERK1-2 (T202-Y204) in HEK293 cells

Data show that treatment of HEK293 cells with Erlotinib inhibits phosphorylation of ERK1/2 at T202/Y204 by EGF.

Z'-factor determination (QC Test) HEK293 cells

The Z’-factor value of 0.6 indicates that the assay is robust and suitable for HTS.

HEK293 control lysate titration (QC Test)

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

Head-to-head kit comparison

Data show a comparable performance between THUNDER™ and the competing TR-FRET kit.

Head-to-head kit comparison

Data show that THUNDER™ outperforms the competing TR-FRET kit.

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