THUNDER™ Total ERK1/2 TR-FRET Cell Signaling Assay Kit

2019-12-12

View technical data sheet View generic user manual

$409.00 – $8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total ERK1/2 (both phosphorylated and non-phosphorylated ERK1/2) in cell lysates. This kit can be used to normalize data obtained with the Phospho-ERK1/2 (T202/Y204) kit and the Extreme Phospho-ERK1/2 (T202/Y204) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: N/A Category: ERK1/2

Size

Clear

Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of PHOSPHO-ERK1-2(T202-Y204) in HEK293 cells

Data show that treatment of HEK293 cells with EGF stimulates phosphorylation of ERK1/2 at T202/Y204, but does not affect the levels of total ERK1/2.

Inhibition of PHOSPHO-ERK1-2 (T202-Y204) in HEK293 cells

Data show that treatment of HEK293 cells with Erlotinib inhibits phosphorylation of ERK1/2 at T202/Y204 by EGF, but does not affect the levels of total ERK1/2.

HEK293 control lysate titration (QC Test)

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Total ERK1/2 TR-FRET Cell Signaling Assay Kit

Additional information

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Total ERK1/2 TR-FRET Cell Signaling Assay Kit

Additional information

Related products

THUNDER™ Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit

THUNDER™ Extreme Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-ERK1/2 (T202/Y204) + Total ERK1/2 TR-FRET Cell Signaling Assay Kit

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS