THUNDER™ Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit

2019-12-12

View technical data sheet View generic user manual

$409.00 – $8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT1/2/3 (S473) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay outperforms the two other TR-FRET assays available. BEST SELLER

SKU: N/A Category: AKT pan

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Stimulation of Phospho-AKT pan (S473) NIH3T3cells

Treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473.

Inhibition Phospho-AKT pan (S473) in NIH3T3 cells

Treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473.

Z'-factor determinationPhospho in NIH3T3 cells

The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR FRET ratio values.

Inhibition Phospho-AKT pan (S473) in Jurkat cells

Treatment of Jurkat cells with LY294002 inhibits phosphorylation of AKT pan at S473.

Data show that the THUNDER™ assay kit outperforms the other two competing TR-FRET kits.

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit

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THUNDER™ Phospho-AKT pan (T308) + Total AKT pan TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-AKT pan (S473) + Total AKT pan TR-FRET Cell Signaling Assay Kit

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS