THUNDER™ Total AKT pan TR-FRET Cell Signaling Assay Kit

2019-12-12

View technical data sheet

$409.00 – $8,390.00

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total AKT 1/2/3 (both phosphorylated and non-phosphorylated AKT1/2/3) in cell lysates. This kit can be used to normalize data obtained with the Phospho-AKT pan (S473) kit and the Phospho-AKT pan (T308) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

SKU: N/A Category: AKT pan

Size

Clear

Additional information

Additional information

Size	100 points, 500 points, 2500 points, 5000 points, 10000 points

Stimulation of Phospho-AKT pan (S473) in NIH3T3 cells

Data show that treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473 but does not affect the levels of total AKT pan.

Inhibition of Phospho-AKT pan (S473) in NIH3T cells

Data show that treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473 by PDGF-AA but does not affect the levels of total AKT pan.

NIH3T3 control lysate titration (QC test)

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Total AKT pan TR-FRET Cell Signaling Assay Kit

Additional information

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET

THUNDER™TR-FRET TOOLBOX REAGENTS

COMPLEMENTARY PRODUCTS

THUNDER™ Total AKT pan TR-FRET Cell Signaling Assay Kit

Additional information

Related products

THUNDER™ Phospho-AKT pan (T308) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-AKT pan (S473) + Total AKT pan TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-AKT pan (T308) + Total AKT pan TR-FRET Cell Signaling Assay Kit

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET

THUNDER™
TR-FRET
TOOLBOX REAGENTS